Publications

Publications 2015

A plug-and-play approach to antibody-based therapeutics via a chemoselective dual click strategy
Maruani, A, Smith, MEB, Miranda, E,  Chester, KA, Chudasama, V, Caddick, S.
Nat Commun, 2015, Accepted.

A mild TCEP-based para-azidobenyl cleavage strategy to transform reversible cysteine thiol labelling reagents into irreversible conjugates.

Maruani, A., Alom, S., Canavelli, P., Lee, M., Morgan R.E., Chudasama, V., Caddick, S.
Chem Commun, 2015, Accepted.

Publications 2014

Next generation maleimides enable the controlled assembly of antibody-drug conjugatesvia native disulfide bond bridging.

Schumacher, F.F., Nunes, J.P.M., Maruani, A., Chudasama, V., Smith, M.E.B., Chester, K.A., Baker, J.R., Caddick, S.
Org Biomol Chem, 2014, 7261-7269.

Regioselective and stoichiometrically controlled conjugation of photodynamic sensitizers to a HER2 targeting antibody fragment.
Bryden, F., Maruani, A., Savoie, H., Chudasama, V., Smith, M.E.B., Caddick, S., Boyle, R.W.
Bioconjug Chem, 2014, 25, 611-617.

Bromo- and thiomaleimides as a new class of thiol-mediated fluorescence ‘turn-on’ reagents.
Youziel, J., Akhbar, A.R., Aziz, Q., Smith, M.E.B., Caddick, S., Tinker, A., Baker, J.R.
Org Biomol Chem, 2014, 12, 557-560.

Publications 2013

Acid-cleavable thiomaleamic acid linker for homogeneous antibody-drug conjugation.

Castañeda, L, Maruani, A, Schumacher, FF, Miranda, E, Chudasama, V, Chester, KA, Baker, JR, Smith, MEB, Caddick, S.
Chem Comm, 2013, 49, 8187-8189.

 

A Mild Synthesis of N-functionalised Bromomaleimides, Thiomaleimide and Bromopyridazinediones.
Castañeda L, Wright ZVF, Marculescu C, Tran, TM, Chudasama V, Maruani A, Hull, EA, Nunes JPM, Fitzmaurice RJ, Smith MEB, Jones LH, Caddick S, Baker JR.
Tetrahedron Lett, 2013, 54, 3493-3495.

 

Homogeneous Antibody Fragment Conjugation by Disulfide Bridging Introduces ‘Spinostics’.
Schumacher F, Sanchania VA, Tolner B, Wright ZVF, Ryan CP, Smith MEB, Ward JM, Caddick S, Kay CWM, Aeppli G, Chester KA, Baker JR.
Sci Rep, 2013, 3, 1525.

 

Reversible Protein Affinity-Labelling Using Bromomaleimide-Based Reagents.
Nathani RI, Chudasama V, Ryan CP, Moody PR, Morgan RE, Fitzmaurice RJ, Smith, MEB, Baker J, Caddick S.
Org Biomol Chem, 2013, 2408-2411.

 

Development of a long acting human growth hormone analog suitable for once a week dosing.

Palanki MS, Bhat A, Bolanos B, Brunel F, Del Rosario J, Dettling D, Horn M, Lappe R, Preston R, Sievers A, Stankovic N, Woodnut G, Chen G.

Biorg. Med. Chem. Lett. 2013, 23, 402-406

 

Work by Pfizer shows protein conjugates incorporating our chemistry are well tolerated and functional in vivo.

 

Publications 2012

Bromomaleimide-linked Bioconjugates are Cleavable in Mammalian Cells.
Moody P, Smith ME, Ryan CP, Chudasama V, Baker JR, Molloy J, Caddick S.
Chembiochem, 2012, 13, 39-41.

 

Highly efficient disulfide bridging polymers for bioconjugates from radical-compatible dithiophenol maleimides.

Jones MW, Strickland RA, Schumacher FF, Caddick S, Baker JR, Gibson MI, Haddleton DM.

Chem Commun (Camb). 2012 Apr 28;48(34):4064-6.

 

 

Polymeric dibromomaleimides as extremely efficient disulfide bridging bioconjugation and pegylation agents.

Jones MW, Strickland RA, Schumacher FF, Caddick S, Baker JR, Gibson MI, Haddleton DM.

J Am Chem Soc. 2012 Jan 25;134(3):1847-52.

 

 

Publications 2011

Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis.
Ryan, C. P., Smith, M. E. B., Schumacher, F. F., Grohmann, D., Papaioannou, D., Waksman, G., Werner, F., Baker, J. R., Caddick, S.
Chem Commun, 2011, 47, 5452-5454. doi:10.1039/C1CC11114K

Bromopyridazinedione-mediated Protein and Peptide Bioconjugation.
Chudasama V, Smith ME, Schumacher FF, Papaioannou D, Waksman G, Baker JR, Caddick S.
Chem Commun, 2011, 47, 8781-8783.

In Situ Maleimide Bridging of Disulfides and a New Approach to Protein PEGylation

Felix F. Schumacher, Muriel Nobles, Chris P. Ryan, Mark E. B. Smith, Andrew Tinker, Stephen Caddick and James R. Baker.

Bioconjugate Chem, 2011, 22, 132.

 

This article is one of the Top 10 most read articles in Bioconjugate Chemistry for 2011.

 

 

Tunable Reagents for Multi-functional Bioconjugation: Reversible or Permanent Chemical Modification of Proteins and Peptides by Control of  Maleimide Hydrolysis

Chris P. Ryan, Mark E. B. Smith, Felix F. Schumacher, Dina Grohmann, Danai Papaioannou, Gabriel Waksman, Finn Werner, James R. Baker and Stephen Caddick.

Chem. Commun., 2011, 47, 5452. For the full article click here.

 

 

Protein Modification, Bioconjugation and Disulfide Bridging using Bromomaleimides

Mark E. B. Smith, Felix F. Schumacher, Christopher P. Ryan, Lauren M. Tedaldi, Danai Papaioannou, Gabriel Waksman, Stephen Caddick* and James R. Baker*

J. Am. Chem. Soc., 2010, 132, 1960.

 

This article was reviewed in both Nature Chemical Biology and Chemistry World.